Protocols
Clearing Immunolabeled Egg Chambers and Embryos

DNA Staining (TOTO3 or OliGreen or DAPI)

Embryo Whole Mount in situ Hybridization with DIG-labeled RNA Probes Followed by Tyramide Signal Amplification

Embryo and Egg Chamber Whole Mount FISH and Immunolabeling

Embryo/Egg Chamber Whole Mount FISH

Formaldehyde/Methanol Fixation for Embryos

Immunohistochemistry

Isolating and Fixing Egg Chambers

Methanol Fixation for Embryos

RNA Probe Synthesis - DIG Conjugated

Fluorophore RNA Labeling by in vitro Transcription

Home